ABSTRACT
Twenty soil communities from the northeastern forests (Assam) and the Western Ghats (Maharashtra) were screened for the presence of the lysine aminotransferase (lat) gene from Nocardia. Hybridization probes and primers were synthesized in accordance with the reported sequence of the Nocardia lat gene from GenBank (number: G1 49355). Seven positives were obtained from the 20 soils. Six of the seven positive were from the Western Ghats and one from the northeast Assam forests. Eighteen actinomycete isolates from the 7 positive soils showed the presence of the lat gene. Only 9 isolates actually produced an antibiotic. These results are discussed.
Subject(s)
Bacterial Proteins/genetics , India , Lysine/metabolism , Molecular Sequence Data , Nocardia/enzymology , Soil Microbiology , Transaminases/geneticsABSTRACT
Se encontró que cepas de Nocardia asteroides y N. brasiliensis poseenn actividad de fosfatasa ácida. Esta se encuentra asociada a las células y aumenta según éstas se reproducen durante su ciclo de crecimiento. De las cepas probadas, N. brasiliensis posee la mayor actividad enzimática. Las implicaciones de estos hallazgos se relacionan a evidencia reciente que sugiere que otras fosfatasas microbianas pueden constituir factores de patogenicidad para los humanos